The Thrombolytic and Cytotoxic Effects of Nigella sativa (L.) Seeds: The Prophetic Medicine

Authors

  • Z. M. Babar International Institute for Halal Research and Training (INHART), International Islamic University Malaysia, Level 3, KICT Building, Jalan Gombak, 53100 Selangor, Malaysia
  • I. Jaswir International Institute for Halal Research and Training (INHART), International Islamic University Malaysia, Level 3, KICT Building, Jalan Gombak, 53100 Selangor, Malaysia
  • M.H.M. Maifiah International Institute for Halal Research and Training (INHART), International Islamic University Malaysia, Level 3, KICT Building, Jalan Gombak, 53100 Selangor, Malaysia
  • S. Ismail Department of Basic Medical Sciences, Faculty of Medicine, International Islamic University Malaysia, Level 3, 53100 Selangor, Malaysia
  • R. A. Raus Department of Biotechnology, Faculty of Engineering, International Islamic University Malaysia, Jalan Gombak, 53100 Selangor, Malaysia
  • A. M. Tareq Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh
  • F. Ahfter Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh
  • A. Faraque School of Food Science and Engineering, South China University of Technology, Wuchan Campus, Building 32, Guangzhou, Guangdong, 510640, China
  • A. S. M. Ali Reza Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh
  • M. A. Sayeed Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh
  • M.M. Hossain Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh
  • M. Uddin Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh
  • M. S. Hossain Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh
  • S. Farhad Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh
  • W. M. Azizi Kolej Universiti Antarabangsa PICOMS (PIUC), Taman Batu Muda, 3, Jalan 31/10a, Taman Perindustrian Iks, 68100 Kuala Lumpur, Wilayah Persekutuan Kuala Lumpur, Malaysia
  • S. J. Arief Faculty of Dentistry, International Islamic University Malaysia, Jalan Hj. Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia.
  • Q. U. Ahmed Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia, Jalan Hj. Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia.
  • I. Mawa Department of Microbiology, University of Science and Technology Chittagong, Zakir Hossain Road, Foy’s Lake, Khulshi, Chittagong 4202, Bangladesh

DOI:

https://doi.org/10.18517/ijhr.2.2.70-77.2020

Keywords:

Brine Shrimp Nauplii (Artemia salina); Cytotoxicity; Human Blood Clot; Thrombolysis; Water-Soluble Extract (WSE)

Abstract

The Water-Soluble Extract (WSE) is a crude bioactive phytoconstituent of Nigella sativa (L.) seeds discovered recently. The current findings report about the thrombolytic and cytotoxic effects of WSE using human blood clot lysis and brine shrimp lethality (BSL) bioassay. The thrombolytic effect of WSE (1,666.67 µg/mL) was determined via the clot and lysate weight measurements compared to streptokinase (STK) of 30,000 IU/mL and normal saline (NS) while the cytotoxicity of WSE (44.14-2,000 µg/mL) against vincristine sulfate (VCS;3.125-100 µg/mL). WSE has shown extremely statistically significant (p<0.0001) clot lysis (90.00%) compared to NS (3.76%) whilst it was also significantly different (p<0.0063) to STK (72.41%) exhibiting LC50 of 1,795.90 µg/mL vs. VCS (39.25 µg/mL) in a dose-dependent manner. The current results suggested WSE has a potent thrombolytic effect with mild dose-dependent cytotoxicity towards brine shrimp nauplii (Artemia salina). It also suggested WSE might have enzymatic roles on thrombin, fibrin, and plasmin of blood. This pharmacological action of WSE is might be due to its antioxidant property, short-chain fatty acids and/or amino acids. Further studies are highly recommended on the enzymatic role(s) and bioactive phytoconstituents of WSE.

Author Biography

I. Jaswir, International Institute for Halal Research and Training (INHART), International Islamic University Malaysia, Level 3, KICT Building, Jalan Gombak, 53100 Selangor, Malaysia

Professor

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Published

2020-12-18

How to Cite

[1]
Z. U. M. Babar, “The Thrombolytic and Cytotoxic Effects of Nigella sativa (L.) Seeds: The Prophetic Medicine ”, Int.J.Halal.Res, vol. 2, no. 2, pp. 70–77, Dec. 2020.

Issue

Vol. 2 No. 2 (2020)

Section

Articles